Mitochondria Antibody

Product Specifications

Background

Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006) . In addition, mitochondria have been shown to play a role in apoptosis (Green 1998) . This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Synonyms

Mitochondrial marker

Host

Mouse

Cross Reactivity

Human

Target

Mitochondria

Clonality

Monoclonal

Isotype

IgG1

Clone

113-1

Conjugation

Unconjugated

Source

Human cell homogenate

Applications

WB, IHC

Purification

Purified by Protein A.

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS

Modification

Unmodified

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Specificity

Specific for endogenous levels of the ~60 kDa human mitochondria. The antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraformaldehyde fixed (5-10 minutes) cryostat sections.