CRISPR Cas9 Antibody

Product Specifications

Background

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015) .

Synonyms

Cas9 antibody, CRISPR-associated endonuclease Cas9/Csn1 antibody, CRISPR-Cas9/Csn1 antibody, csn1 antibody, SpyCas9 antibody

Host

Mouse

Cross Reactivity

Others

Target

CRISPR Cas9

Clonality

Monoclonal

Isotype

IgG1

Clone

6F7

Conjugation

Unconjugated

Source

Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.

Applications

WB, IHC

Purification

Purified by Protein G.

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 50% glycerol and 5 mm NaN3

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.