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Actin Antibody

Product Specifications

Background

Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

Synonyms

A actin antibody, ACTA antibody, ACTA1 antibody, Actin alpha skeletal muscle antibody, Actin antibody, actin alpha 1 skeletal muscle 1 antibody, actin alpha 1 skeletal muscle antibody, Actin alpha skeletal muscle antibody, actina antibody, actine antibody, ACTS_HUMAN antibody, aktin antibody, Alpha Actin 1 antibody, Alpha skeletal muscle Actin antibody, alpha skeletal muscle antibody, alpha-actin antibody, Alpha-actin-1 antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, nemaline myopathy type 3 antibody

Gene ID

421534

Swiss Prot

P68139

Host

Mouse

Cross Reactivity

Human, Mouse, Rat, All

Target

Actin

Clonality

Monoclonal

Isotype

IgG1

Clone

C4

Conjugation

Unconjugated

Source

Preparation of chicken gizzard actin

Applications

WB, IHC

Purification

Mouse ascites fluid

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IHC ()

Buffer

Ascites fluid + 0.01% NaN3.

Modification

Unmodified

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Specificity

Specific for endogenous levels of the ~42 kDa actin protein. Reacts with all actin isoforms.

Gene ID URL

421534

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