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Phospho-Nucleolin (Thr76) Recombinant Antibody, PE-Cy5.5 Conjugated

Product Specifications

Background

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Synonyms

MS1116; NCL; Nucl; Nucleolin; Protein C23.

Gene ID

4691

Swiss Prot

P19338

Modification Site

Thr76

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human

Target

Nucleolin

Clonality

Recombinant

Isotype

IgG

Conjugation

PE-Cy5.5

Source

KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T76

Applications

WB

Purification

Purified by Protein A.

Excitation Emission

488nm/694nm

Concentration

Lot dependent

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

4691

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