Phospho-Nucleolin (Thr84) Recombinant Antibody, AbBy Fluor™ 488 Conjugated
Product Specifications
Background
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Synonyms
NCL; Nucl; Nucleolin; Protein C23.
Gene ID
4691
Swiss Prot
P19338
Modification Site
Thr84
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Nucleolin
Clonality
Recombinant
Isotype
IgG
Conjugation
AbBy Fluor™ 488
Source
KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T84
Applications
WB, IF
Purification
Purified by Protein A.
Excitation Emission
499nm/519nm
Concentration
Lot dependent
Dilution
WB (1:300-5000), IF ()
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
4691
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