Anti-CIDE-A (CT) Antibody

Rabbit Polyclonal Antibody specific to CIDE-A (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Cell death-inducing DFFA-like effector A

Gene Name

Cidea

Gene ID

12683

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Cidea">Cidea</a>

UniProt

O70302

Accession Number

NP_031728

Cellular Locus

Lipid droplet. Nucleus. Note=Enriched at lipid droplet contact sites. Using a GFP-tagged construct, has been shown to localize to mitochondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (PubMed:12910269). These data could not be confirmed (PubMed:22245780, PubMed:18509062).

Host

Rabbit

Reactivity

Mouse

Immunogen

Peptide corresponding to aa 200-214 of mouse CIDE-A (accession no. AAC34985).

Target Antigen

Cell death activator CIDE-A

Target

CIDE-A (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. {PubMed:18509062, PubMed:22144693, PubMed:22245780, PubMed:9564035}.

Additionnal Information

Immunoblotting: use at 2ug/mL. Immunohistochemistry:use at 5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Tissue lysate of mouse heart.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes mouse CIDE-A (25kDa) and does not cross-react with CIDE-B.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

DFF45-related proteins CIDE-A and CIDE-B have recently been identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.