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RhoA/B/C Recombinant Antibody, Cy5.5 Conjugated

Product Specifications

Background

RhoA , RhoB , RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Synonyms

ARH12, ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC.

Gene ID

387

Swiss Prot

P61586, P62745, P08134

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

RhoA/B/C

Clonality

Recombinant

Isotype

IgG

Conjugation

Cy5.5

Source

KLH conjugated synthetic peptide derived from human RhoA

Applications

WB, IF

Purification

Purified by Protein A.

Excitation Emission

675nm/694nm

Concentration

Lot dependent

Dilution

WB (1:300-5000), IF ()

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

387

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