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Phospho-Erk1 (T202) +Erk2 (T185) Monoclonal Antibody, Cy3 Conjugated

Product Specifications

Background

The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].

Gene ID

5595

Swiss Prot

P27361

Modification Site

Thr202+Thr185

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-Erk1 (T202) +Erk2 (T185)

Clonality

Monoclonal

Isotype

IgG

Clone

6H2

Conjugation

Cy3

Source

Synthetic phospho-Peptide corresponding to residues surrounding Thr185 of human Erk2.

Applications

WB, IF, Flow-Cyt

Purification

Purified by Protein A.

Excitation Emission

512,550nm/570,615nm

Concentration

Lot Dependent

Dilution

WB (1:300-5000), IF (), Flow-Cyt (1ug/Test)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

5595

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