H3K27ac (1A3) Monoclonal Antibody
Product Specifications
Background
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called histone code. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Host
Mouse
Cross Reactivity
Human
Target
H3K27ac
Clonality
Monoclonal
Isotype
IgG1
Clone
1A3
Conjugation
Unconjugated
Source
Monoclonal antibody raised in mouse against histone H3 acetylated at lysine 27 (H3K27ac), using a KLHconjugated synthetic peptide.
Applications
WB, ELISA, IF (ICC)
Purification
Protein A purified monoclonal antibody in PBS containing 0.05% azide.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
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