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PPAR alpha (7G5) Monoclonal Antibody

Product Specifications

Background

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) . Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

Synonyms

PPAR; NR1C1; hPPAR; PPARalpha; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1; PPARA

Gene ID

5465

Swiss Prot

Q07869

Host

Mouse

Cross Reactivity

Human, Mouse

Target

PPAR alpha

Clonality

Monoclonal

Isotype

IgG1

Clone

7G5

Conjugation

Unconjugated

Source

This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.

Applications

WB, IHC-P, IHC-F, IF, ICC/IF

Purification

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Concentration

0.5µg/µl

Dilution

WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF (), ICC/IF (1:50-100)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Gene ID URL

5465

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