Myc Tag Monoclonal Antibody
Product Specifications
Background
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. The anti-Myc Tag monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.
Host
Mouse
Cross Reactivity
Species independent
Target
Myc Tag
Clonality
Monoclonal
Isotype
IgG1
Clone
9E10
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide encoding c-Myc tag was used as antigen.
Applications
WB
Purification
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Concentration
0.5µg/µl
Dilution
WB (1:300-5000)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
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