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Myc Tag Monoclonal Antibody

Product Specifications

Background

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. The anti-Myc Tag monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.

Host

Mouse

Cross Reactivity

Species independent

Target

Myc Tag

Clonality

Monoclonal

Isotype

IgG1

Clone

9E10

Conjugation

Unconjugated

Source

KLH conjugated synthetic peptide encoding c-Myc tag was used as antigen.

Applications

WB

Purification

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Concentration

0.5µg/µl

Dilution

WB (1:300-5000)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

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