Bcl-2 monoclonal Antibody, APC Conjugated
Product Specifications
Background
The Bcl-2 gene was isolated at the chromosomal breakpoint of t (14;18) -bearing follicular B cell lymphomas (1,2) .Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5) .Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7) .A more recently identified protein, designated Bax p21 (i.e., Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2 (8) . Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2 (8) .
Synonyms
Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
Gene ID
596
Swiss Prot
P10415
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Mouse
Cross Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Horse, Guinea Pig
Immunogen
51-150/239
Target
Bcl-2
Clonality
Monoclonal
Isotype
IgG
Clone
4H1
Conjugation
APC
Source
KLH conjugated synthetic peptide derived from human Bcl-2
Applications
WB, Flow-Cyt
Purification
Purified by Protein G.
Excitation Emission
650nm/660nm
Concentration
1µg/µl
Dilution
WB (WB=1:500-2000), Flow-Cyt (Flow-Cyt=1:100)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
596
Curated Selection
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