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Phosphotyrosine Monoclonal Antibody, RBITC Conjugated

Product Specifications

Background

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Synonyms

Phospho-tyrosine; Phospho tyrosine; Phospho-tyr; Phospho tyr; p-Tyr; p-tyrosine

Modification Site

Phosphoserine

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Phosphotyrosine

Clonality

Monoclonal

Isotype

IgG

Clone

4D3

Conjugation

RBITC

Source

KLH conjugated Phosphotyrosine

Applications

IF (ICC), IF

Purification

Purified by Protein G.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

IF (ICC) (1:50-200), IF ()

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

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