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ECFP (10H5) Monoclonal Antibody, AbBy Fluor™ 488 Conjugated

Product Specifications

Background

Cyan Fluorescent Protein (CFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. The fluorescence of EYFP was detected with an excitation filter of 485 nm and an emission filter of 545 nm, and that of ECFP was detected with filters of 420 and 486 nm (Perkin Elmer), respectively. In the present reporter assay, EYFP and ECFP were selected as a reporter protein and an internal control for normalization of transfection, respectively.

Synonyms

ECFPTag; ECFPTag; tagECFP; Cyan Fluorescent Protein; Enhanced Cyan Fluorescent Protein; ECFP-Tag.

Host

Mouse

Cross Reactivity

Species independent

Target

ECFP

Clonality

Monoclonal

Isotype

IgG

Clone

10H5

Conjugation

AbBy Fluor™ 488

Source

Recombinant ECFP

Applications

WB

Purification

Purified by Protein G.

Excitation Emission

499nm/519nm

Concentration

1µg/µl

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

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