AmCyan (5F9) Monoclonal Antibody, Cy3 Conjugated
Product Specifications
Background
AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively) . DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD) . The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.
Host
Mouse
Cross Reactivity
Species independent
Target
AmCyan
Clonality
Monoclonal
Isotype
IgG
Clone
5F9
Conjugation
Cy3
Source
Recombinant AmCyan
Applications
WB
Purification
Purified by Protein G.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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