TAP-Tag Monoclonal Antibody, Cy5.5 Conjugated
Product Specifications
Background
Tandem affinity purification (TAP) is a purification technique for studying proteinprotein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.
Synonyms
TAP Tag; TAPTag; Tandem affinity purification
Host
Mouse
Cross Reactivity
Species independent
Target
TAP-Tag
Clonality
Monoclonal
Isotype
IgG
Clone
4F7
Conjugation
Cy5.5
Source
Recombinant TAP-Tag
Applications
WB
Purification
Purified by Protein A.
Excitation Emission
675nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.biossantibodies.com/datasheets/bsm-33133M-Cy5.5
Cross Reactivity Details
TAP-Tag
Curated Selection
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