Histone H3 (di methyl K36) Monoclonal Antibody, RBITC Conjugated

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms

Histone Cluster 3, H3; H3 Histone Family, Member T; Histone 3, H3; H3FT; H3/G; H3/T; H3t; H3.4 ; Histone H3.1t ; HIST3H3; HGNC:4778; H31T_HUMAN

Gene ID

8290

Swiss Prot

P68431

Cellular Locus

Nucleus

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Histone H3 (di methyl K36)

Clonality

Monoclonal

Isotype

IgG1

Clone

3F7

Conjugation

RBITC

Source

KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of di methyl K36

Applications

WB, IF (IHC-P), IF (ICC)

Purification

Purified by Protein G.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

8290

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