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Histone H3 (mono methyl K9) Monoclonal Antibody, Cy5 Conjugated

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Synonyms

Histone Cluster 3, H3; H3 Histone Family, Member T; Histone 3, H3; H3FT; H3/G; H3/T; H3t; H3.4 ; Histone H3.1t ; HIST3H3; HGNC:4778; H31T_HUMAN

Gene ID

P68431

Swiss Prot

8290

Cellular Locus

Nucleus

Host

Mouse

Cross Reactivity

Human, Mouse, Rat

Target

Histone H3 (mono methyl K9)

Clonality

Monoclonal

Isotype

IgG

Clone

3F8

Conjugation

Cy5

Source

KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of mono methyl K9

Applications

WB, IF

Purification

Purified by Protein G.

Excitation Emission

625,650nm/670nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF ()

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

P68431

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