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Ki-67 Monoclonal Antibody, FITC Conjugated

Product Specifications

Background

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase) . It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index) . In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Synonyms

Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki-67; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.

Gene ID

4288

Swiss Prot

P46013

Host

Mouse

Cross Reactivity

Human

Target

Ki-67

Clonality

Monoclonal

Isotype

IgG

Clone

6B9

Conjugation

FITC

Source

KLH conjugated synthetic peptide derived from human Ki-67

Applications

FCM, IF

Purification

Purified by Protein G.

Excitation Emission

494nm/518nm

Concentration

1µg/µl

Dilution

FCM (1:20-100), IF ()

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

4288

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