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RFP Monoclonal Antibody, Cy3 Conjugated

Product Specifications

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.

Synonyms

GFP like chromoprotein; red fluorescent protein; Red fluorescent protein eqFP611; RFP; RFPTag; RFP Tag.

Host

Mouse

Cross Reactivity

Species independent

Target

RFP

Clonality

Monoclonal

Isotype

IgG

Clone

7A5

Conjugation

Cy3

Source

Recombinant Red fluorescent protein

Applications

WB

Purification

Purified by Protein G.

Excitation Emission

512,550nm/570,615nm

Concentration

1µg/µl

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

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