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His tag (6E6) Monoclonal Antibody, AbBy Fluor™ 488 Conjugated

Product Specifications

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Synonyms

HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.

Host

Mouse

Cross Reactivity

Species independent

Target

His tag

Clonality

Monoclonal

Isotype

IgG

Clone

6.00E+06

Conjugation

AbBy Fluor™ 488

Source

Human, fusion protein HHHHHH tagged

Applications

WB

Purification

Purified by Protein G.

Excitation Emission

499nm/519nm

Concentration

1µg/µl

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

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