P47/Pleckstrin Polyclonal Antibody, HRP Conjugated
Product Specifications
Background
Platelet P47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.
Synonyms
P47; Platelet 47 kDa protein; Platelet and leukocyte C kinase substrate; Platelet P47; Platelet p47 protein; Pleckstrin; PLEK; PLEK_HUMAN; FLJ27168; OTTHUMP00000159962.
Gene ID
5341
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
21-120/350
Target
P47/Pleckstrin
Clonality
Polyclonal
Isotype
IgG
Conjugation
HRP
Source
KLH conjugated synthetic peptide derived from human Pleckstrin/p47
Applications
WB, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
5341
Curated Selection
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