Goat anti Human C-reactive protein

The reactivity of the antiserum is restricted to C-reactive protein. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal Human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C-reactive protein. No reaction is obtained with any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C-reactive protein in Human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.

C-reactive protein is an acute phase protein, synthesized in the liver. Its molecular weight is about 140,000. It occurs in serum of healthy men in low concentrations, <1.2 μg/mL. In cases of bacterial infection, surgical trauma and Hodgkins disease, he concentration is highly increased. It may reach values of 0.5 mg/mL. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Precipitation assays. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifµgation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Delipidated, heat inactivated, lyophilized, stable whole antiserum; No preservative added; Total protein and IgG concentrations in the antiserum are comparable to those of pooled; Normal goat serum; No foreign proteins added._x000D_ Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.