Phospho-LEF1 (Ser42) Polyclonal Antibody
Product Specifications
Background
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity) . Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Synonyms
LEF-1; TCF10; TCF7L3; TCF1ALPHA; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; LEF1
Gene ID
51176
Swiss Prot
Q9UJU2
Modification Site
Ser42
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-LEF1 (Ser42)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42
Applications
WB, IHC-P, IHC-F, IF
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), IHC-P (1:200-400), IHC-F (1:100-500), IF ()
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Specificity
This antibody will recognize LEF1 (Ser40) in mouse and rat
Gene ID URL
51176
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
JH495460 1 Each
(2R,3R,4S,5S,6R) -2-[ (2R,3S,4R,5R,6R) -6-decoxy-4,5-dihydroxy-2- (hydroxymethyl) oxan-3-yl]oxy-6- (hydroxymethyl) oxane-3,4,5-triol
Ask
View Details 