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5 MethylCytosine Polyclonal Antibody, AbBy Fluor™ 594 Conjugated

Selected Variant

bs-9450R-BF594

Size: 100 µL

Product Specifications

Background

Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs) to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.

Synonyms

5-Methyl Cytidine; 5 m C; 5 mC; 5 me C; 5 Me Cytidine; 5 MeCyd; 5 Methyl Cytidine; 5 MethylCytosine; 5-mC; 5-Me Cytidine; 5-Methyl-Cytosine; 5-MethylCytidine.

Host

Rabbit

Cross Reactivity

Human, Others

Target

5 MethylCytosine

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 594

Source

KLH conjugated 5 MethylCytosine

Applications

WB, FCM, IF (IHC-P)

Purification

Purified by Protein A.

Excitation Emission

590nm/617nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Cross Reactivity Details

5 MethylCytosine

Price

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