Phospho-Smad2/Smad3 (Thr8) Polyclonal Antibody, Cy5 Conjugated

Product Specifications

Background

SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF) -beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.

Synonyms

Smad2 + Smad3 (phospho T8) ; p-Smad2 + Smad3 (phospho T8) ; P-Smad2 /3 (phospho T8) ; hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3.

Gene ID

4087

Swiss Prot

P84022

Modification Site

Thr8

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-Smad2/Smad3 (Thr8)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5

Source

KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8

Applications

WB, FCM, IF (IHC-P), IF (ICC), IF

Purification

Purified by Protein A.

Excitation Emission

625,650nm/670nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (ICC) (1:50-200), IF ()

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

4087

Predicted Cross Reactivity

Rat, Dog, Cow, Pig, Horse, Chicken

Available Sizes

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