3-Nitrotyrosine Polyclonal Antibody, AbBy Fluor™ 555 Conjugated

Product Specifications

Background

Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.

Synonyms

NULL

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Species independent

Target

3-Nitrotyrosine

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 555

Source

KLH conjugated to Nitrotyrosine

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

553nm/568nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Cross Reactivity Details

Nitrotyrosine

Available Sizes

Curated Selection

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