Luciferase Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Product Specifications

Background

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Synonyms

Luciferin 4-monooxygenase

Gene ID

7100

Cellular Locus

Cytoplasm

Host

Rabbit

Immunogen

101-220/550

Target

Luciferase

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated synthetic peptide derived from Firefly Luciferase

Applications

IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1µg/µl

Dilution

IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

7100

Predicted Cross Reactivity

Firefly

Cross Reactivity Details

Photinus pyralis (Common eastern firefly)

Available Sizes

Curated Selection

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