Luciferase Polyclonal Antibody, AbBy Fluor™ 488 Conjugated
Product Specifications
Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Synonyms
Luciferin 4-monooxygenase
Gene ID
7100
Cellular Locus
Cytoplasm
Host
Rabbit
Immunogen
101-220/550
Target
Luciferase
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 488
Source
KLH conjugated synthetic peptide derived from Firefly Luciferase
Applications
IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
499nm/519nm
Concentration
1µg/µl
Dilution
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
7100
Predicted Cross Reactivity
Firefly
Cross Reactivity Details
Photinus pyralis (Common eastern firefly)
Available Sizes
More Discoveries
Explore Other Products
Browse additional items from our catalog
K0802304 1.0 µg DNA
FRA2F sgRNA CRISPR Lentivector (Human) (Target 3)
Sign In for Pricing
View Details 