Luciferase Polyclonal Antibody, AbBy Fluor™ 405 Conjugated
Product Specifications
Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Synonyms
Luciferin 4-monooxygenase
Gene ID
7100
Cellular Locus
Cytoplasm
Host
Rabbit
Immunogen
101-220/550
Target
Luciferase
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 405
Source
KLH conjugated synthetic peptide derived from Firefly Luciferase
Applications
IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
401nm/421nm
Concentration
1µg/µl
Dilution
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
7100
Predicted Cross Reactivity
Firefly
Cross Reactivity Details
Photinus pyralis (Common eastern firefly)
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