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Nucleolin/C23 Polyclonal Antibody, PerCP Conjugated

Product Specifications

Background

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Synonyms

C23; Nucleolin; Protein C23; NCL

Gene ID

4691

Swiss Prot

P19338

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Rat

Immunogen

601-710/710

Target

Nucleolin/C23

Clonality

Polyclonal

Isotype

IgG

Conjugation

PerCP

Source

KLH conjugated synthetic peptide derived from human Nucleolin

Applications

FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

482nm/677nm

Concentration

1µg/µl

Dilution

FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

4691

Predicted Cross Reactivity

Mouse, Dog, Cow, Pig, Horse

Curated Selection

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