Phospho-Cdc25B (Ser149) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity) .
Synonyms
AI604853; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; Cdc25b; Cdc25m2
Gene ID
12531
Swiss Prot
P30306
Modification Site
Ser149
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-Cdc25B (Ser149)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from mouse Cdc25B around the phosphorylation site of Ser149
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
12531
Predicted Cross Reactivity
Human, Rat
Curated Selection
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