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Phospho-Cdc25B (Ser149) Polyclonal Antibody, AbBy Fluor™ 488 Conjugated

Product Specifications

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity) .

Synonyms

AI604853; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; Cdc25b; Cdc25m2

Gene ID

12531

Swiss Prot

P30306

Modification Site

Ser149

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Mouse

Target

Phospho-Cdc25B (Ser149)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 488

Source

KLH conjugated synthetic phosphopeptide derived from mouse Cdc25B around the phosphorylation site of Ser149

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

499nm/519nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

12531

Predicted Cross Reactivity

Human, Rat

Curated Selection

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