Lin28 Polyclonal Antibody, Biotin Conjugated

Product Specifications

Background

LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II.

Synonyms

Protein lin-28 homolog A; LIN28A; Lin-28A; Zinc finger CCHC domain-containing protein 1; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog B; LIN28B; Lin-28B; CSDD2

Gene ID

79727, 389421

Swiss Prot

9H9Z2, Q6ZN17

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Mouse, Rat

Immunogen

75-180/209

Target

Lin28

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Source

KLH conjugated synthetic peptide derived from human Lin28A

Applications

WB, ELISA, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C for 12 months.

Specificity

This antibody is produced using synthetic peptide that falls within the range of 90-120 amino acids of human Lin28A. Due to 92% sequence similarity, this antibody may cross react with Lin28B.