Separase Polyclonal Antibody, AbBy Fluor™ 555 Conjugated
Product Specifications
Background
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]) .[supplied by OMIM, Nov 2010].
Synonyms
Caspase like protein ESPL1; ESP 1; ESP1; ESP-1; ESPL 1; ESPL1; ESPL-1; Extra spindle poles like 1; Extra spindle poles like 1 protein; Separin; Similar to fission yeast cut1and gene; SSE; Separase; ESPL1_HUMAN.
Gene ID
9700
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Immunogen
1851-1950/2120
Target
Separase
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 555
Source
KLH conjugated synthetic peptide derived from human Separase
Applications
IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
553nm/568nm
Concentration
1µg/µl
Dilution
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
9700
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
041448-FITC 200 µL
SCAP, NT (SCAP, KIAA0199, Sterol regulatory element-binding protein cleavage-activating protein) (FITC) discontinued
Ask
View Details 
36-2202-100 100 µg
Anti-EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (Clone: GFR/1667)
Ask
View Details