Phospho-REC8 (Ser251) Polyclonal Antibody, FITC Conjugated
Product Specifications
Background
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
Synonyms
Mrec; Rec8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p; Rec8; Mei8
Gene ID
56739
Swiss Prot
Q8C5S7
Modification Site
Ser251
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-REC8 (Ser251)
Clonality
Polyclonal
Isotype
IgG
Conjugation
FITC
Source
KLH conjugated synthetic phosphopeptide derived from mouse REC8 around the phosphorylation site of Ser251
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
494nm/518nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
56739
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