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Phospho-REC8 (Ser251) Polyclonal Antibody, Cy5 Conjugated

Product Specifications

Background

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

Synonyms

Mrec; Rec8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p; Rec8; Mei8

Gene ID

56739

Swiss Prot

Q8C5S7

Modification Site

Ser251

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Mouse

Target

Phospho-REC8 (Ser251)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5

Source

KLH conjugated synthetic phosphopeptide derived from mouse REC8 around the phosphorylation site of Ser251

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

625,650nm/670nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

56739

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