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Phospho-REC8 (Ser251) Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Product Specifications

Background

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

Synonyms

Mrec; Rec8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p; Rec8; Mei8

Gene ID

56739

Swiss Prot

Q8C5S7

Modification Site

Ser251

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Mouse

Target

Phospho-REC8 (Ser251)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated synthetic phosphopeptide derived from mouse REC8 around the phosphorylation site of Ser251

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

56739

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