Phospho-REC8 (Ser251) Polyclonal Antibody, APC-Cy5.5 Conjugated
Product Specifications
Background
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.
Synonyms
Mrec; Rec8L1; Meiotic recombination protein REC8 homolog; Cohesin Rec8p; Rec8; Mei8
Gene ID
56739
Swiss Prot
Q8C5S7
Modification Site
Ser251
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-REC8 (Ser251)
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC-Cy5.5
Source
KLH conjugated synthetic phosphopeptide derived from mouse REC8 around the phosphorylation site of Ser251
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
56739
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