Rad23 Polyclonal Antibody, HRP Conjugated
Product Specifications
Background
The RAD23 gene of Saccharomyces cerevisiae is required for excision-repair of UV damaged DNA (1) . RAD23 resembles the other DNA repair genes, RAD2, RAD6, RAD7, RAD18, and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis (2) . RAD23 encodes a nuclear protein containing a ubiquitin-like domain required for biological function (3) . RAD23 bears a ubiquitin-like domain at its amino terminus and this ubiquitin-like domain affects protein function in a nonproteolytic manner (3) . Rad23 is a highly conserved protein involved in nucleotide excision repair (NER) that associates with the proteasome via its amino-terminus (4) . Its carboxy-terminal ubiquitin-associated domain is evolutionarily conserved from yeast to humans (4) . In addition to a role in DNA repair events in yeast, several lines of evidence indicate that the Rad23 protein may regulate the activity of the 26 S proteasome (5) .
Synonyms
DNA repair protein RAD23; UV excision repair protein RAD23; RAD23_YEAST; YEL037C; SYGP-ORF29.
Gene ID
856674
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Mouse
Immunogen
51-150/398
Target
Rad23
Clonality
Polyclonal
Isotype
IgG
Conjugation
HRP
Source
KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae Rad23
Applications
WB, ELISA, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
856674
Predicted Cross Reactivity
Yeast
Cross Reactivity Details
Saccharomyces cerevisiae
Curated Selection
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