N-WASP Antibody
Product Specifications
Background
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types
Synonyms
Neural Wiskott-Aldrich syndrome protein, WASL, WASP
Swiss Prot
O00401
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Unphosphorylated N-WASP (Ser-484/Ser-485)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Unphosphorylated N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP.
Applications
WB
Purification
Antigen Affinity purification
Dilution
WB (1:300-5000)
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Modification
Unmodified
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Product Datasheet
https://www.biossantibodies.com/datasheets/bs-70728R
Specificity
This antibody was cross-adsorbed to phospho-N-WASP (S484/S485) peptide (without carrier) then Affinity purification using unphosphorylated N-WASP (S484/S485) peptide (without carrier) .
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