TRPM7 (Ser-1493), Phosphospecific Antibody

Product Specifications

Background

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.

Synonyms

TRPM7 TrpC7, LTrpC-7, ChaK1, LTRPC7, TRP

Swiss Prot

Q96QT4

Modification Site

Ser-1493

Host

Rabbit

Cross Reactivity

Human

Target

TRPM7 (Ser-1493)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Phospho-TRPM7 (Ser-1493) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1493 in human TRPM7.

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.