NSMase3 (C-terminal region) Antibody

Product Specifications

Background

Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependence. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase3 (also known as SMPD4) is highly expressed in skeletal muscle and heart tissues, and is expressed in many cancer cell lines. The nSMase3 protein is localized to endoplasmic reticulum and Golgi, and may be upregulated in response to DNA damage and cell stress.

Synonyms

SMPD4, neutral sphingomyelinase 3, nSMase sphingomyelin

Swiss Prot

Q6ZPR5

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

NSMase3 (C-terminal region)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

NSMase3 synthetic peptide corresponds to amino acids in the C-terminal region of mouse nSMase3.

Applications

WB, IP

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000), IP (1-2ug)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects an 85-90 kDa* band corresponding to nSMase3 on SDS-PAGE immunoblots of adult mouse heart and mouse C2C12 cells. The antibody works for western blot, ELISA, immunoprecipitation, and immunocytochemistry. This sequence is highly conserved in rat and human nSMase3, and has low homology to other nSMase family members.