Slingshot-1L (C-terminal region) Antibody

Product Specifications

Background

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.

Synonyms

SSH1L

Swiss Prot

Q8WYL5

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Slingshot-1L (C-terminal region)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1L.

Applications

WB, IHC

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 150 kDa* protein corresponding to the molecular mass of SSH1L on SDS-PAGE immunoblots of PC12 cells. This sequence has 100% homology with similar regions of mouse and bovine SSH1L, and has low homology to SSH2L and SSH3L.