Semaphorin-3A (N-terminal) Antibody

Product Specifications

Background

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

Synonyms

Sema3A, Semaphorin III

Swiss Prot

Q14563

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Semaphorin-3A (N-terminal)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Semaphorin 3A synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminal region of human Sema3A.

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 95kDa* protein corresponding to the apparent molecular mass of Sema3A on SDS-PAGE immunoblots of human recombinant Sema3A/Fc chimera. This antibody has not been shown to be reactive in neonatal rat brain, but has weak reactivity in adult mouse brain. The sequence used is identical to similar regions in rat and mouse Sema3A, and has low homology to other semaphorin family members.