Paxillin (Thr-538), Phosphospecific Antibody

Product Specifications

Background

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at tyrosine, threonine, and serine sites. Serine and threonine phosphorylation of paxillin occur in response to growth-factor activation, PKC activators, and fibronectins. Phosphorylation of Ser-85, Ser-178, and Thr-538 may be important sites for regulating paxillin activity. Paxillin phosphorylation of Thr-538 occurs in response to TPA-activated PKCs in vitro, and this phosphorylation may contribute to dissolution of the actin cytoskeleton and redistribution of LFA-1 integrins in vivo.

Swiss Prot

P49023

Modification Site

Thr-538

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat, Chicken, Xenopus

Target

Paxillin (Thr-538)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Phospho-Paxillin (Thr-538) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 538 from human paxillin a.

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. This sequence is highly conserved in rat and mouse paxillin and is also found in the other paxillin isoforms (b & g) .