Paxillin (Ser-178), Phosphospecific Antibody

Product Specifications

Background

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration

Swiss Prot

P49023

Modification Site

Ser-178

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Paxillin (Ser-178)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Phospho-Paxillin (Ser-178) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin.

Applications

WB, IHC

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells. This human sequence is highly conserved in rat and mouse paxillin.