Neuropilin-1 (a1 CUB Domain) Antibody

Product Specifications

Background

Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 has been implicated as a receptor involved in axon guidance and VEGF signaling. NRP-1 mediates activation of intracellular signaling pathways through interaction with its co-receptors, Plexin-A1 and VEGFRs. The expression of NRP-1, along with the co-receptor Plexin-A1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

Synonyms

NRP1, VEGF 165, VEGFR

Swiss Prot

O14786

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Neuropilin-1 (a1 CUB Domain)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Neuropilin-1 synthetic peptide (coupled to carrier protein) corresponding to amino acids within the a1 CUB domain of human neuropilin-1.

Applications

WB, IHC

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody detects a 130 kDa* protein corresponding to the apparent molecular mass of neuropilin-1 on SDS-PAGE immunoblots of COS-7 cells transfected with mouse neuropilin-1. In immunocytochemistry, this antibody co-localizes with anti-HA in Cos-7 cells transfected with HA-tagged mouse neuropilin-1. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.