Myosin 13/Extraocular Myosin (Hinge region) Antibody

Product Specifications

Background

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11) .

Synonyms

Myh13, myosin heavy chain

Swiss Prot

B1AR69

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Myosin 13/Extraocular Myosin (Hinge region)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH13/extraocular myosin.

Applications

WB, IHC

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000), IHC ()

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Unmodified

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH13 on SDS-PAGE immunoblots of mouse extraocular muscle, and does not detect MYHs in mouse diaphragm, gastrocnemius, or brain. This sequence has 100% identity with rat and human MYH13, and has low homology to other myosin family members.