Myosin IIA Heavy Chain (Ser-1943), Phosphospecific Antibody

Product Specifications

Synonyms

NMHC-IIA, MYH9, myosin heavy chain

Swiss Prot

P35579

Modification Site

S1943

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat, Chicken

Target

Myosin IIA Heavy Chain (Ser-1943), phospho-specific

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Phospho-NMHC-IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain.

Applications

Purification

Antigen Affinity purification

Dilution

WB (1:300-5000)

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Modification

Phosphorylation

Storage Conditions

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Specificity

This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier) . The antibody detects a 200 kDa* protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.